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Hyper production of cellulase-free xylanase by Thermomyces ...

Jan 01, 2009· A cellulase-free xylanase production by Thermomyces lanuginosus −1 −1 dried carbon source) after 120 h with 10% inoculum. The biobleaching efficacy of crude xylanase was tested on bagasse pulp, and the maximum brightness of 46.1 ± 0.06% was observed with 50 U of crude xylanase per gram of pulp, which was 3.8 points higher than the brightness of untreated …

Optimization of Xylanase Production by Thermomyces ...

Extracellular xylanase production by the thermo-philic fungus Thermomyces lanuginosus 195 in solid state fermentation (SSF) was found to be significantly affected by fermentation temperature, duration, and inoculum volume (p 0:001). Optimization of these parameters corresponded to a 21.7% increase in xylanase yield. Maximum activity (2,335U/g ...

(PDF) Sorghum straw for xylanase hyper-production by (DW ...

Download Free PDF. Sorghum straw for xylanase hyper-production by (DW) under solid-state fermentation. Bioresource Technology, 2005. Bhupinder Chadha. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper. READ PAPER.

Hyper production of cellulase-free xylanase by Thermomyces ...

A cellulase-free xylanase production by Thermomyces lanuginosus SSBP using bagasse pulp was examined under submerged (SmC) and solid-state cultivation (SSC). Higher level of xylanase activity (19,320 +/- 37 U g(-1) dried carbon source) was obtained in SSC cultures than in SmC (1,772 +/- 15 U g(-1) dried carbon source) after 120 h with 10% inoculum.

(PDF) Optimized Expression of a Thermostable Xylanase from ...

Download Free PDF. Optimized Expression of a Thermostable Xylanase from Thermomyces lanuginosus in Pichia pastoris. Applied and Environmental Microbiology, 2003. Orlando Martins. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper.

Hyper production of cellulase-free xylanase by Thermomyces ...

Jan 01, 2009· 10%· A cellulase-free xylanase production by Thermomyces lanuginosus −1 −1 dried carbon source) after 120 h with 10% inoculum. The biobleaching efficacy of crude xylanase was tested on bagasse pulp, and the maximum brightness of 46.1 ± 0.06% was observed with 50 U of crude xylanase per gram of pulp, which was 3.8 points higher than …

New cellulase-free xylanase enzyme - produced by culturing ...

New exocellulase- and endocellulase-free xylanase (I) is produced by culturing Thermomyces lanuginosus on a nutrient medium contg. lignocellulose. The T. lanuginosus strain used is specifically DSM 58

Hyper production of cellulase-free xylanase by Thermomyces ...

Sep 19, 2008· A cellulase-free xylanase production by Thermomyces lanuginosus SSBP using bagasse pulp was examined under submerged (SmC) and solid-state cultivation (SSC). Higher level of xylanase activity (19,320 +/- 37 U g(-1) dried carbon source) was obtained in SSC cultures than in SmC (1,772 +/- 15 U g(-1) dried carbon source) after 120 h with 10% inoculum.

Alkali-thermostable and cellulase-free xylanase production ...

Sep 13, 2006· The optimization of cultural variables resulted in a marked enhancement in the secretion of cellulase-free and alkali-thermostable xylanase (EC 3.2.1.8) by an extreme thermophile Geobacillus thermoleovorans. The enzyme secretion was enhanced when the medium was supplemented with xylan (0.15%) and Tween-80 (0.1% v/v). In wheat bran …

Production of ᅫᄇ-xylanase by a Thermomyces lanuginosus …

xylanase(EC3.2.1.8),andβ-xylosidase(EC3.2.1.37)(4).Xylanasesand the associated debranching enzymes are produced by a variety of mic-robes, including bacteria, yeast, and filamentous fungi (2, 5).Among them, Thermomyces lanuginosus (thermophilic fungus) has been re-ported as one of the superior cellulase-free xylanase producers (6).

High level expression of a recombinant xylanase by Pichia ...

Jul 27, 2011· Hyper production of cellulase-free xylanase by Thermomyces lanuginosus SSBP on bagasse pulp and its application in biobleaching. Manimaran A, Kumar KS, Permaul K, Singh S. Appl Microbiol Biotechnol, 81(5):887-893, 19 Sep 2008 Cited by: 12 articles | PMID: 18802693. Production of beta-xylanase by a Thermomyces lanuginosus MC 134 mutant on corn ...

Cellulase-free xylanase by Thermomyces lanuginosus RT9 ...

Several carbohydrates were examined for their ability to induce xylanase in Thermomyces lanuginosus RT9 at 50°C in shake flask cultures. It was found that xylan or xylan containing substrates, such as corn cobs, induced maximum and comparable level of xylanase free of cellulase, while the easily metabolisable sugars (glucose, xylose, and sucrose) appeared to …

User Publications | Bioinformatics Solutions Inc.

Tryptic Mapping Based Structural Insights of Endo-1, 4-β-Xylanase from Thermomyces lanuginosus VAPS-24: 09:16:19: 2020/05/19: Marker peptide combination for source identification of gelatins obtained from Equidae hides by LC–MS/MS detection: 10:21:00: 2020/05/19

BRENDA - Information on EC 3.2.1.8 - endo-1,4-beta-xylanase

Hyper production of cellulase-free xylanase by Thermomyces lanuginosus SSBP on bagasse pulp and its application in biobleaching Appl. Microbiol. Biotechnol. 81 887-893 2009 Thermomyces lanuginosus 18802693. ... cellulase-free xylanase from deep-sea Kocuria sp. Mn22 J. Microbiol. Biotechnol. 19 873-880 2009 Kocuria sp. Mn22 (B8XY24) 19809242.

β-xylanase from Thermomyces lanuginosus and its ...

The ability of T. lanuginosus in which produced large amount of high thermos stable xylanase has made this fungus to be a source of xylanase production for biobleaching in pulp and paper process. How to cite this article: K. Khucharoenphaisan and K. Sinma, 2010. β-xylanase from Thermomyces lanuginosus and its Biobleaching Application.

Cellulase-free xylanase by thermophilic fungi: a ...

10%· Thermomyces lanuginosus strains RT9 and MH4 were studied to find favourable cultivation conditions and to compare their abilities to produce xylanolytic enzymes in three media on different substrates at 50° C or 55° C under shake-culture conditions. Both organisms produced xylanases free of cellulase at widely different levels in all cultivation …

Characterization of a novel endo-β-1,4-glucanase from ...

Xylanase Induction Catabolite repression White-rot fungi a b s t r a c t This paper reports regulation of endoglucanase (EC 3.2.1.4) and xylanase (EC 3.2.1.8) production in …

(PDF) Gamma Irradiation enhancement for cellulase free ...

PDF | On Sep 1, 2010, Kairia A. Yousef and others published Gamma Irradiation enhancement for cellulase free-xylanase production by different …

Optimized Expression of a Thermostable Xylanase from ...

Dec 17, 2020· Highly efficient production of a Thermomyces lanuginosus IOC-4145 β-1,4-xylanase was achieved in Pichia pastoris under the control of the AOX1 promoter. P. pastoris colonies expressing recombinant xylanase were selected by enzymatic activity plate assay, and their ability to secrete high levels of the enzyme was evaluated in small-scale cultures. …

Hyper production of cellulase-free xylanase by Thermomyces ...

A cellulase-free xylanase production by Thermomyces lanuginosus SSBP using bagasse pulp was examined under submerged (SmC) and solid-state cultivation (SSC). Higher level of xylanase activity (19,320 ± 37 U g−1 dried carbon source) was obtained in SSC cultures than in SmC (1,772 ± 15 U g−1 dried carbon source) after 120 h with 10% inoculum. The biobleaching …

(PDF) Bleach boosting effects of purified xylanase from ...

Purified xylanase from T. lanuginosus SSBP was sup- plied by Lin et al. [13]. The enzyme displayed pH and 2.5. Alkali extraction and elemental chlorine-free temperature optima of 6.5 and 65 °C, respectively, with bleaching a molecular mass of 23.6 kDa and a pI value of 3.8.

A bifunctional cellulase–xylanase of a new ...

Dec 04, 2017· It is the first bifunctional cellulase–xylanase reported in GH5_46 as well as in Chryseobacterium genus. The enzyme showed an endoglucanase activity on carboxymethylcellulose of 3237 μmol min −1 mg −1 at pH 9, 90 °C and a xylanase activity on birchwood xylan of 1793 μmol min −1 mg −1 at pH 8, 90 °C. The activity level and ...

Production and properties of the cellulase-free xylanase ...

Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition

Cellulase-free xylanase by Thermomyces lanuginosus RT9 ...

Sep 15, 1994· Several carbohydrates were examined for their ability to induce xylanase in Thermomyces lanuginosus RT9 at 50°C in shake flask cultures. It was found that xylan or xylan containing substrates, such as corn cobs, induced maximum and comparable level of xylanase free of cellulase, while the easily metabolisable sugars (glucose, xylose, and sucrose) …

Characterization of a cellulase-free, neutral xylanase ...

Aug 01, 2005· A xylanase purified from the thermophilic fungus Thermomyces lanuginosus CBS 288.54 was characterized and its potential application in wheat straw pulp biobleaching was evaluated. Xylanase was purified 33.6-fold to homogeneity with a recovery yield of 21.5%. It appeared as a single protein band on SDS-PAGE gel with a molecular mass of approx. 26.2 kDa.

Novel perspectives for evolving enzyme cocktails for ...

Kumar R, Wyman CE: Effects of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies. Bioresour Technol 2009,100:4203-4213. Lynd LR, Laser MS, Bransby D, Dale BE, Davidson B, Hamilton R, Himmel ME, Keller M, McMillan JD, Sheehan J: How biotech can transform biofuels.

Hyper production of cellulase-free xylanase by …

A cellulase-free xylanase production by Thermomyces lanuginosus SSBP using bagasse pulp was examined under submerged (SmC) and solid-state cultivation (SSC). Higher level of xylanase activity ...

How to buy Characterization of a cellulase-free, neutral ...

Characterization of a cellulase-free, neutral xylanase from Thermomyces lanuginosus CBS 288.54 and its biobleaching effect on wheat straw pulp [An article from: Bioresource Technology], provided by Elsevier, has been proved to be very good at present. Here we introduce how to purchase this quality product.

Thermostable xylanases from thermophilic fungi and ...

Apr 01, 2019· Thermomyces lanuginosus with optimum growth temperature of 50˚C is considered as one of the most prolific producer of alkaline active virtually cellulase free thermostable xylanases that exhibited 3500 (units/ml) and 48,000 (units/g substrate) of activity under shake flask and solid substrate fermentation, respectively (Sonia et al., 2005 ...

Hyper production of cellulase-free xylanase by …

A cellulase-free xylanase production by Thermomyces lanuginosus SSBP using bagasse pulp was examined under submerged (SmC) and solid-state cultivation (SSC). Higher level of xylanase activity (19,320 +/- 37 U g(-1) dried carbon source) was obtained in SSC cultures than in SmC (1,772 +/- 15 U g(-1) dried carbon source) after 120 h with 10% inoculum.

Review: Engineering of thermostable enzymes for industrial ...

Jan 11, 2018· 1 Biomolecular Engineering Lab, Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy; 2 Center for Nano Science and Technology at Polimi, Istituto Italiano di Tecnologia, Via G. Pascoli 70/3, 20133 Milano, Italy; a) Author to whom correspondence should be addressed: [email …

Purification and characterization of a novel cellulase ...

Jun 01, 2003· Abstract. A β-xylanase (XynIII) of Acrophialophora nainiana was purified to homogeneity from the culture supernatant by ultrafiltration and a combination of ion exchange and gel filtration chromatographic methods. It was optimally active at 55°C and pH 6.5. XynIII had molecular masses of 27.5 and 54 kDa, as estimated by gel filtration and sodium dodecyl …

US8986977B2 - Disruption of cell walls for enhanced lipid ...

Presented herein are methods of using cell wall degrading enzymes for recovery of internal lipid bodies from biomass sources such as algae. Also provided are algal cells that express at least one exogenous gene encoding a cell wall degrading enzyme …

cellulase free xylanase by thermomyces

Cellulase-free xylanase by Thermomyces lanuginosus RT9. Several carbohydrates were examined for their ability to induce xylanase in Thermomyces lanuginosus RT9 at 50°C in shake flask cultur It was found that xylan or xylan containing substrates, such as corn cobs, induced maximum and comparable level of xylanase free of cellulase, while the easily metabolisable …

Production and characterization of thermostable xylanases ...

Apr 01, 1994· Thermomyces lanuginosus and Thermoascus aurantiacus isolated from self-heated jute fiber stalk were studied for xylanase production using various lignocelluloses under solid-state fermentation. Both organisms performed creditably well with unsupplemented wheat bran at 55°C. T. lanuginosus produced cellulase-free xylanase, whereas T. aurantiacus …

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